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KMID : 0984920130150020105
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Journal of Skin Barrier Research
2013 Volume.15 No. 2 p.105 ~ p.105
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The Dietary Effect of Royal Jelly Supplementation for the Pre and Post -Menopaused Period on Epidermal Levels of Filaggrin, Free Amino Acids and the Related Enzyme Expression in Rats
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Kim Yea-Ji
Han Sang-Mi
Cho Yun-Hi
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Abstract
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Epidermal hydration is mainly maintained by natural moisturizing factors (NMFs). Of various NMFs, free amino acids (AAs) are major constituents that are generated by filaggrin degradation and its reduction is reported in the aged skin induced by menopause. In this study, we examined whether dietary supplementation of royal jelly (RJ) during pre- and post menopausal period alters epidermal levels of filaggrins, free AAs and peptidylarginine deiminase-3 (PAD-3), an enzyme involved in filaggrin degradation processes. Sprague Dawley rats were divided into five groups: groups SHAM and OVX (ovariectomy) fed a control diet; groups RJ1 and RJ2 fed a diet with 1% RJ harvested in different area of Korea; group IF fed a diet with isoflavone (80mg/kg), the typical functional food for menopause prevention for 12 weeks. Ovariectomy was performed at 4 weeks and each diet was continued for 8 more weeks. In the epidermis of group OVX, total filaggrin (including profilaggrin and filaggrin) were reduced; these levels in groups RJ1 and IF were similar or less than in group OVX, but total AAs, of which alteration were not apparent between groups SHAM and OVX, were highly increased. Specifically aspartate (Asp) and proline (Pro), the major AAs functioning NMF, were highly increased in group RJ1. Although total filaggrin, profilaggrin, filaggrin and PAD3 were increased, total AAs (including Asp and Pro) in group RJ2 were modestly less than in group RJ1. The PAD3 alteration was not apparent among 4 other groups. The diet supplementation of RJ1 enhanced the filaggrin degradation, but not by increased protein expression of PAD3, along with the increased total AAs, Asp and Pro.
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